报告摘要：A novel strategy to finely control a large metabolic flux by using a “metabolic transistor” approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The “metabolic transistor” strategy was applied to controlling electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The 4-hydroxybenzoate geranyltransferase (lePGT-1, encoded by the lepgt gene) from Lithospermum erythrorhizon can competitively consume substrates required for the biosynthesis of ubiquinone-8 (Q8). Therefore, flexible regulation of LA production can be achieved by controlling the expression level of the lepgt gene to precisely control the flux of the ETC. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy. This strategy was also applied to the regulation of the molar percentage (mol%) of the LA fraction in P(3HB-co-LA) biosynthesis.

主讲人简介：

吴辉，华东理工大学生物工程学院教授、博导。入选国家高层次青年人才，教育部“霍英东青年教师基金” 获得者。华东理工大学发酵工程博士、莱斯大学生物工程系和中科院合成生物学重点实验室博士后，主要从事微生物低碳代谢工程与代谢调控研究。主持国家自然科学基金面上/青年项目、国家重点研发计划课题/子课题等项目。Biotechnol Adv、Metab Eng、ACS Sustain Chem Eng、Bioresource Technol、ACS Synth Biol等期刊发表论文60余篇，出版专著3部。曾先后获宝钢优秀教师奖，侯德榜化工技术奖青年奖、上海市教学成果奖一等奖、中国石油与化工教育教学优秀成果一等奖。目前担任中国轻工业生物基材料工程重点实验室副主任，中国生物工程学会青年工作委员会委员，中国生物工程学会一碳生物技术专委会委员，上海市生物工程学会一碳生物技术专委会副主任委员，上海市生物工程学会合成生物学委员会委员，生物反应器工程国家重点实验室固定人员。Front Microbiol副主编、J Ind Microbiol Biot、J Chem Technol Biot、Front Bioeng Biotechnol编委和Bioresource Bioprocess青年编委。